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Staff information

Galya

Galya Orr

Biosystms Dynamics & Simula
Biomedical Scientist
Pacific Northwest National Laboratory
PO Box 999
MSIN: K8-88
Richland, WA 99352

Biography

Dr. Orr is the capability lead for the cell isolation and systems analysis (CISA), where she leads the team in the development and applications of capabilities for biological research at the molecular level. She has been initiating and leading interdisciplinary efforts that combine the development of non-conventional quantitative fluorescence imaging and molecular biology approaches and their applications to better understand cellular and molecular processes in the intact cell. Building on her experience in electrophysiology of learning and memory, she combined single-molecule measurements to investigate dynamics - function relationships of membrane receptors. Since then, her research has been focused on molecular level understanding of information transfer across the cell membrane, initiated by physiological cues or environmental challenges.

Experience:

2010-present: Senior Research Scientist-Team Lead, Pacific Northwest National Lab, Richland, WA 2003-2010: Senior Research Scientist, Pacific Northwest National Laboratory, Richland, WA 2000-2003: Research Scientist, Pacific Northwest National Laboratory, Richland, WA 1999-2000: Graduate Fellow, Pacific Northwest National Laboratory, Richland, WA 1996-1999: Graduate Research Associate, Neural Systems Memory & Aging, University of Arizona, Tucson 1994-1995: Graduate Research Assistant, Bekesy Laboratory of Neurobiology, University of Hawaii 1987-1993: Research Specialist, Arizona Research Laboratories, Division of Neurobiology, U of Arizona 1985-1987: Research Associate, Pacific International Center for Technology Research, U of Hawaii

Research Interests

  • Dr. Orr has been the lead PI on a grant funded by NIH-NIEHS, on a STAR grant funded by the EPA, and on a grant from the Air Force Research Laboratory, where quantitative super resolution and single-molecule flourescence imaging and molecular biology techniques have been applied to investigate the cell surface interactions and intracellular fate of nanomaterials. She has been part of the NIEHS consortium and Oregon Nanoscience and Microtechnologies Institute team that focus on delineating mechanisms of nanoparticle toxicity or biocompatibility, with the ultimate goal of predicting and guiding the development and applications of safer nanomaterials.

Education and Credentials

  • Ph.D - Neuroscience, Division of Neural Systems Memory and Aging, University of Arizona, Tucson (2002)
  • Toward Ph.D - Cell Molecular & Neuroscience, University of Hawaii at Manoa, Honolulu, Hawaii (1995)
  • B.A - Molecular and Cellular Biology, Israel Institute of Technology, The Technion, Haifa, Israel (1984)

Affiliations and Professional Service

  • Peer Reviewer and Advisory Boards:
  • 2012: NSF - Onsite Review Panel - Duke University - Center for the Environmental Implications of Nano-Technology
  • Peer reviewer for Biophysical Journal, ChemBioChem, ACS Nano, Nanotoxicology, Advance Materials, Toxicology and Applied Pharmacology, Toxicological Sciences, International Journal of Biomedical Nanoscience and Nanotechnology, Chemical Research in Toxicology, Bentham Science Publishers - Advances in Drug Delivery, The Journal of Microscopy, The European Journal of Clinical Investigation, American Journal of Physiology - Lung Cellular and Molecular Physiology, Journal of Proteome Research. Proposals reviewer for DOE SBIR/STTR Program, NSF Early Career Program, DOE BES Energy Biosciences Research Program, Ohio Agricultural Research and Development Center SEED Program, Netherlands Organization for Scientific Research - Division for Chemical Sciences, NSF EPSCoR program.
  • 2012: NSF - External Advisory Board - Uof Wisconsin-Madison -Nanoscale Science and Engineering Center
  • 2011: NSF advisory workshop - Nanomaterials and the Environment: Chemistry and Materials Perspective
  • 2010: EPA - Panel reviewer - Fate, Transport and Transformation of Nanomaterials
  • 2010: Co-Chair - Nanotoxicology at the 50th Society of Toxicology Annual Meeting
  • Society of Toxicology - since 2006
  • Biophysical Society - since 2001
  • Society for Neuroscience - since 1996

Awards and Recognitions

  • 2010: Outstanding Mentor Award, US Department of Energy, Office of Science
  • 2010: Fitzner-Eberhardt Award Nominee for Outstanding Contributions to Science and Engineering Education, Pacific Northwest National Laboratory (PNNL)
  • 2009- NIEHS - consortium member - Nanotechnology Health Implications Research
  • 2009: NIH/NIEHS - Panel reviewer - Superfund Basic Research Program (SBRP)
  • 2009: Significant Contributor Award, Environmental Biomarkers Initiative, PNNL
  • 2008: Outstanding Performance Award, PNNL
  • 2007- Oregon Nanoscience and Microtechnologies Institute - Faculty member
  • 2000: Key Contributor Award, Biomolecular Networks Initiative, PNNL
  • 1996-1997: Academic Achievements Merit Fellowship, University of Arizona
  • 1994-1995: Academic Achievements Merit Scholarship, University of Hawaii
  • 1983-1984: Dean's list of Honors for scholastic achievements, Israel Institute of Technology Professional Societies and Service

PNNL Publications

2018

  • Cui Y., D. Hu, L. Markillie, W.B. Chrisler, M.J. Gaffrey, C.K. Ansong, and L. Sussel, et al. 2018. "Fluctuation localization imaging-based fluorescence in situ hybridization (fliFISH) for accurate detection and counting of RNA copies in single cells." Nucleic Acids Research 46, no. 2:Article No. e7. PNNL-SA-126413. doi:10.1093/nar/gkx874
  • Daly P., S.C. Lopez, M. Peng, C.S. Lancefield, S.O. Purvine, Y. Kim, and E.M. Zink, et al. 2018. "Dichomitus squalens partially tailors its molecular responses to the composition of solid wood." Environmental Microbiology 20, no. 11:4141-4156. PNNL-SA-135900. doi:10.1111/1462-2920.14416
  • Melby E.S., Y. Cui, J. Borgatta, A.C. Mensch, M. Hang, W.B. Chrisler, and A. Dohnalkova, et al. 2018. "Impact of lithiated cobalt oxide and phosphate nanoparticles on rainbow trout gill epithelial cells." Nanotoxicology 12, no. 10:1166-1181. PNNL-SA-137427. doi:10.1080/17435390.2018.1508785

2017

  • Howard-Varona C., S. Roux, H. Dore, N.E. Solonenko, K.M. Holmfeldt, L.M. Markillie, and G. Orr, et al. 2017. "Regulation of infection efficiency in a globally abundant marine Bacteriodetes virus." The ISME Journal 11, no. 1:284-295. PNNL-SA-115144. doi:10.1038/ismej.2016.81
  • Lemmer K.C., W. Zhang, S.J. Langer, A. Dohnalkova, D. Hu, R.A. Lemke, and J.S. Piotrowski, et al. 2017. "Mutations that alter the bacterial cell envelope increase lipid production." mBio 8, no. 3:Article No. e00513-17. PNNL-SA-125854. doi:10.1128/mBio.00513-17
  • Orellana R., G. Chaput, L. Markillie, H.D. Mitchell, M.J. Gaffrey, G. Orr, and K.M. Deangelis. 2017. "Multi-time series RNA-seq analysis of Enterobacter lignolyticusSCF1 during growth in lignin-amended medium." PLoS One 12, no. 10:e0186440. PNNL-SA-131719. doi:10.1371/journal.pone.0186440

2016

  • Ford N.R., K.A. Hecht, D. Hu, G. Orr, Y. Xiong, T. Squier, and G.L. Rorrer, et al. 2016. "Antigen Binding and Site-Directed Labeling of Biosilica-Immobilized Fusion Proteins Expressed in Diatoms." ACS Synthetic Biology 5, no. 3:193-199. PNNL-SA-113297. doi:10.1021/acssynbio.5b00191
  • Hua X., C.J. Szymanski, Z. Wang, Y. Zhou, X. Ma, J. Yu, and J.E. Evans, et al. 2016. "Chemical imaging of molecular changes in a hydrated single cell by dynamic secondary ion mass spectrometry and super-resolution microscopy." Integrative Biology 8, no. 5:579-666. PNNL-SA-110602. doi:10.1039/c5ib00308c
  • Melby E.S., A.C. Mensch, S.E. Lohse, D. Hu, G. Orr, C. Murphy, and R.J. Hamers, et al. 2016. "Formation of supported lipid bilayers containing phase-segregated domains and their interaction with gold nanoparticles." Environmental Science: Nano 3, no. 1:45-55. PNNL-SA-111557. doi:10.1039/C5EN00098J
  • Mitchell H.D., L. Markillie, W.B. Chrisler, M.J. Gaffrey, D. Hu, C.J. Szymanski, and Y. Xie, et al. 2016. "Cells Respond to Distinct Nanoparticle Properties with Multiple Strategies as Revealed by Single-Cell RNA-Seq." ACS Nano 10, no. 11:10173-10185. PNNL-SA-121323. doi:10.1021/acsnano.6b05452
  • Proetto M.T., C.R. Anderton, D. Hu, C.J. Szymanski, Z. Zhu, J.P. Patterson, and J.K. Kammeyer, et al. 2016. "Cellular Delivery of Nanoparticles Revealed with Combined Optical and Isotopic Nanoscopy." ACS Nano 10, no. 4:4046-4054. PNNL-SA-106955. doi:10.1021/acsnano.5b06477
  • Zhang J., G.N. Presley, K.E. Hammel, J.S. Ryu, J. Menke, M. Figueroa, and D. Hu, et al. 2016. "Localizing gene regulation reveals a staggered wood decay mechanism for the brown rot fungus Postia placenta." Proceedings of the National Academy of Sciences of the United States of America 113, no. 39:10968-10973. PNNL-SA-114266. doi:10.1073/pnas.1608454113

2015

  • Dominguez G.A., S.E. Lohse, M. Torelli, C. Murphy, R.J. Hamers, G. Orr, and R.D. Klaper. 2015. "Effects of charge and surface ligand properties of nanoparticles on oxidative stress and gene expression within the gut of Daphnia magna." Aquatic Toxicology 162. PNNL-SA-108642. doi:10.1016/j.aquatox.2015.02.015
  • Geng T., E.L. Bredeweg, C.J. Szymanski, B. Liu, S.E. Baker, G. Orr, and J.E. Evans, et al. 2015. "Compartmentalized microchannel array for high-throughput analysis of single cell polarized growth and dynamics." Scientific Reports 5. PNNL-SA-109268. doi:10.1038/srep16111
  • Grate J.W., K. Mo, Y. Shin, A. Vasdekis, M.G. Warner, R.T. Kelly, and G. Orr, et al. 2015. "Alexa Fluor-labeled Fluorescent Cellulose Nanocrystals for Bioimaging Solid Cellulose in Spatially Structured Microenvironments." Bioconjugate Chemistry 26, no. 3:593-601. PNNL-SA-88815. doi:10.1021/acs.bioconjchem.5b00048
  • Jacobson K.H., I.L. Gunsolus, T.R. Kuech, J.M. Troiano, E.S. Melby, S.E. Lohse, and D. Hu, et al. 2015. "Lipopolysaccharide density and structure govern the extent and distance of nanoparticle interaction with actual and model bacterial outer membranes." Environmental Science & Technology 49, no. 17:10642-10652. PNNL-SA-112154. doi:10.1021/acs.est.5b01841
  • Mihai C., W.B. Chrisler, Y. Xie, D. Hu, C.J. Szymanski, A. Tolic, and J. Klein, et al. 2015. "Intracellular accumulation dynamics and fate of zinc ions in alveolar epithelial cells exposed to airborne ZnO nanoparticles at the air-liquid interface." Nanotoxicology 9, no. 1:9-22. PNNL-SA-99125. doi:10.3109/17435390.2013.859319
  • Murphy C., A.M. Vartanian, F.M. Geiger, R.J. Hamers, J.A. Pedersen, Q. Cui, and C.L. Haynes, et al. 2015. "Biological Responses to Engineered Nanomaterials: Needs for the Next Decade." ACS Central Science 1, no. 3:117-123. PNNL-SA-113908. doi:10.1021/acscentsci.5b00182
  • Pomraning K.R., S. Wei, S.A. Karagiosis, Y. Kim, A. Dohnalkova, B.W. Arey, and E.L. Bredeweg, et al. 2015. "Comprehensive metabolomic, lipidomic and microscopic profiling of Yarrowia lipolytica during lipid accumulation identifies targets for increased lipogenesis." PLoS One 10, no. 4:e0123188. PNNL-SA-105704. doi:10.1371/journal.pone.0123188
  • Sontag R.L., C. Mihai, G. Orr, A. Savchenko, T. Skarina, H. Cui, and J.R. Cort, et al. 2015. "Electroporation of Functional Bacterial Effectors into Mammalian Cells." Journal of Visualized Experiments 95. PNNL-SA-102481. doi:10.3791/52296
  • Szymanski C.J., P. Munusamy, C. Mihai, Y. Xie, D. Hu, M.K. Gilles, and T. Tyliszczak, et al. 2015. "Shifts in oxidation states of cerium oxide nanoparticles detected inside intact hydrated cells and organelles." Biomaterials 62. PNNL-SA-110620. doi:10.1016/j.biomaterials.2015.05.042
  • Troiano J., L.L. Olenick, T.R. Kuech, E.S. Melby, D. Hu, S.E. Lohse, and A.C. Mensch, et al. 2015. "Direct Probes of 4 nm Diameter Gold Nanoparticles Interacting with Supported Lipid Bylayers." Journal of Physical Chemistry C 119, no. 1:534-546. PNNL-SA-102945. doi:10.1021/jp512107z

2014

  • Bishop L.M., A.S. Tillman, F.M. Geiger, C.L. Haynes, R.D. Klaper, C. Murphy, and G. Orr, et al. 2014. "Enhancing Graduate Student Communication to General Audiences through Blogging about Nanotechnology and Sustainability." Journal of Chemical Education 91, no. 10:1600-1605. PNNL-SA-106817. doi:10.1021/ed500050d
  • Croken M., Y. Ma, L.M. Markillie, R.C. Taylor, G. Orr, L.M. Weiss, and K. Kim. 2014. "Distinct Strains of Toxoplasma gondii Feature Divergent Transcriptomes Regardless of Developmental Stage." PLoS One 9, no. 11:Article No. e111297. PNNL-SA-104591. doi:10.1371/journal.pone.0111297
  • Eraso J.M., L.M. Markillie, H.D. Mitchell, R.C. Taylor, G. Orr, and W. Margolin. 2014. "The highly conserved MraZ protein is a transcriptional regulator in Escherichia coli." Journal of Bacteriology 196, no. 11:2053-2066. PNNL-SA-98128. doi:10.1128/JB.01370-13
  • Gunsolus I.L., D. Hu, C. Mihai, S.E. Lohse, C. Lee, M. Torelli, and R.J. Hamers, et al. 2014. "Facile method to stain the bacterial cell surface for super-resolution fluorescence microscopy." Analyst 139, no. 12:3174-3178. PNNL-SA-102724. doi:10.1039/c4an00574k
  • Tilton S.C., N.J. Karin, A. Tolic, Y. Xie, X. Lai, R.F. Hamilton, and K.M. Waters, et al. 2014. "Three Human Cell Types Respond to Multi-Walled Carbon Nanotubes and Titanium Dioxide Nanobelts with Cell-Specific Transcriptomic and Proteomic Expression Patterns." Nanotoxicology 8, no. 5:533-548. PNNL-SA-94058. doi:10.3109/17435390.2013.803624
  • Welkie D.G., X. Zhang, L.M. Markillie, R.C. Taylor, G. Orr, J.M. Jacobs, and K. Bhide, et al. 2014. "Transcriptomic and proteomic dynamics in the metabolism of a diazotrophic cyanobacterium, Cyanothece sp. PCC 7822 during a diurnal light-dark cycle." BMC Genomics 15. PNNL-SA-104956.

2013

  • Chien M., A.S. Carlini, D. Hu, C.V. Barback, A.M. Rush, D.J. Hall, and G. Orr, et al. 2013. "Enzyme-Directed Assembly of Nanoparticles in Tumors Monitored by In Vivo Whole Animal and Ex Vivo Super-Resolution Fluorescence Imaging." Journal of the American Chemical Society 135, no. 50:18710-18713. PNNL-SA-98263. doi:10.1021/ja408182p
  • Hu D., B. Zhao, Y. Xie, G. Orr, and A.D. Li. 2013. "Understanding Super-Resolution Nanoscopy and Its Biological Applications in Cell Imaging." Physical Chemistry Chemical Physics. PCCP 15, no. 36:14856-14861. PNNL-SA-95464. doi:10.1039/C3CP51629F
  • Kleese van Dam K., J.P. Carson, A.L. Corrigan, D.R. Einstein, Z.C. Guillen, B.S. Heath, and A.P. Kuprat, et al. 2013. "Velo and REXAN - Integrated Data Management and High Speed Analysis for Experimental Facilities." In Proceedings of the IEEE 8th International Conference on EScience, October 8-12, 2012, Chicago, Illinois. Los Alamitos, California:IEEE Press. PNNL-SA-89470. doi:10.1109/eScience.2012.6404463
  • Nakayasu E.S., R.N. Brown, C. Ansong, M.A. Sydor, S. Imtiaz, C. Mihai, and R.L. Sontag, et al. 2013. "Multi-omic data integration links Deleted in Breast Cancer 1 (DBC1) Degradation to Chromatin Remodeling in Inflammatory Response." Molecular & Cellular Proteomics. MCP 12, no. 8:2136-2147. PNNL-SA-88392. doi:10.1074/mcp.M112.026138
  • Welkie D.G., D.M. Sherman, W.B. Chrisler, G. Orr, and L.A. Sherman. 2013. "Analysis of carbohydrate storage granules in the diazotrophic cyanobacterium Cyanothece sp. PCC 7822." Photosynthesis Research 118, no. 1-2:25-36. PNNL-SA-98888. doi:10.1007/s11120-013-9941-z
  • Xia T., R.F. Hamilton, J.C. Bonner, E.D. Crandall, A.C. Elder, F. Fazlollahi, and T.A. Girtsman, et al. 2013. "Interlaboratory Evaluation of in Vitro Cytotoxicity and Inflammatory Responses to Engineered Nanomaterials: The NIEHS Nano GO Consortium." Environmental Health Perspectives 121, no. 6:683-690. PNNL-SA-96811. doi:10.1289/ehp.1306561

2012

  • Karakoti A.S., P. Munusamy, K.E. Hostetler, V.K. Kodali, S.N. Kuchibhatla, G. Orr, and J.G. Pounds, et al. 2012. "Preparation and Characterization Challenges to Understanding Environmental and Biological Impacts of Ceria Nanoparticles." Surface and Interface Analysis 44, no. 8:881-889. PNNL-SA-83765. doi:10.1002/sia.5006
  • Xie Y., N.G. Williams, A. Tolic, W.B. Chrisler, J.G. Teeguarden, B.L. Maddux, and J.G. Pounds, et al. 2012. "Aerosolized ZnO nanoparticles induce toxicity in alveolar type II epithelial cells at the air-liquid interface." Toxicological Sciences 125, no. 2:450-461. PNNL-SA-83610. doi:10.1093/toxsci/kfr251

2011

  • Ahring B.K., N.S. Baliga, J.R. Frederickson, S. Kaplan, H.B. Pakrasi, J.G. Pounds, and I. shah, et al. 2011. Biological Interactions and Dynamics Science Theme Advisory Panel (BID-STAP). PNNL-20717. Richland, WA: Pacific Northwest National Laboratory.
  • Arey B.W., V. Shutthanandan, Y. Xie, A. Tolic, N.G. Williams, and G. Orr. 2011. "Investigation of Cellular Interactions of Nanoparticles by Helium Ion Microscopy." In Scanning Microscopies 2011: Advanced Microscopy Technologies for Defense, Homeland Security, Forensic, Life, Environmental, and Industrial Sciences, April 26, 2011, Orlando, Florida. Proceedings of the SPIE, edited by MT Postek, et al, 8036, Paper No. 80360K. Bellingham, Washington:SPIE. PNNL-SA-78874. doi:10.1117/12.887141
  • Baer D.R., A.S. Karakoti, P. Munusamy, B.D. Thrall, J.G. Pounds, J.G. Teeguarden, and J.E. Amonette, et al. 2011. "Testing in EHS: What is the Current Status of Experimentation?." In 11th IEEE International Conference on Nanotechnology (IEEE-NANO 2011), August 15-18, 2011, Portland, Oregon, 18-19. Piscataway, New Jersey:IEEE. PNNL-SA-90027. doi:10.1109/NANO.2011.6144671
  • Brown G.E., A. Chaka, D.K. Shuh, E.E. Roden, C.J. Werth, N.J. Hess, and A.R. Felmy, et al. 2011. EMSL Geochemistry, Biogeochemistry and Subsurface Science-Science Theme Advisory Panel Meeting. PNNL-20601. Richland, WA: Pacific Northwest National Laboratory.
  • Lea A.S., B.C. Kabius, B.W. Arey, L. Kovarik, C.M. Wang, G. Orr, and I. Lyubinetsky, et al. 2011. "Quietly Building Capabilities: New Instruments, Expertise, "Quiet Wing" Available at DOE User Facility." Microscopy Today 19, no. 5:48-53. PNNL-SA-81095. doi:10.1017/S1551929511000964
  • Orr G., W.B. Chrisler, K.J. Cassens, R. Tan, B.J. Tarasevich, L.M. Markillie, and R.C. Zangar, et al. 2011. "Cellular Recognition and Trafficking of Amorphous Silica Nanoparticles by Macrophage Scavenger Receptor A." Nanotoxicology 5, no. 3:296-311. PNNL-SA-68643. doi:10.3109/17435390.2010.513836

2010

  • Grate J.W., C. Zhang, T.W. Wietsma, M.G. Warner, N.C. Anheier, B.E. Bernacki, and G. Orr, et al. 2010. "A note on the visualization of wetting film structures and a nonwetting immiscible fluid in a pore network micromodel using a solvatochromic dye." Water Resources Research 46. PNNL-SA-71572. doi:10.1029/2010WR009419
  • Hinderliter P.M., K.R. Minard, G. Orr, W.B. Chrisler, B.D. Thrall, J.G. Pounds, and J.G. Teeguarden. 2010. "ISDD: A Computational Model of Particle Sedimentation, Diffusion and Target Cell Dosimetry for In Vitro Toxicity Studies." Particle and Fibre Toxicology 7, no. November:Article No. 36. PNNL-SA-74873. doi:10.1186/1743-8977-7-36
  • Hu D., and G. Orr. 2010. "Nanometer Resolution Imaging by SIngle Molecule Switching." Nano Reviews 1. PNNL-SA-70811. doi:10.3402/nano.v1i0.5122
  • Orr G. 2010. "Organelle-Specific Pharmaceutical Nanotechnology: Active Cellular Transport of Submicro- and Nanoscale Particles." In Organelle-Specific Pharmaceutical Nanotechnology, edited by V Weissig and GG D'Souza. 337-356. Hoboken, New Jersey:John Wiley & Sons. PNWD-SA-8443.
  • Yantasee W., R.D. Rutledge, W. Chouyyok, V. Sukwarotwat, G. Orr, C.L. Warner, and M.G. Warner, et al. 2010. "Functionalized Nanoporous Silica for Removal of Heavy Metals from Biological Systems; Adsorption and Application." ACS Applied Materials & Interfaces 2, no. 10:2749-2758. PNNL-SA-73483. doi:10.1021/am100616b

2009

  • Orr G., D.J. Panther, K.J. Cassens, J.L. Phillips, B.J. Tarasevich, and J.G. Pounds. 2009. "Syndecan-1 Mediates the Coupling of Positively Charged Submicrometer Amorphous Silica Particles with Actin Filaments across the Alveolar Epithelial Cell Membrane." Toxicology and Applied Pharmacology 236, no. 2:210-220. PNWD-SA-8493. doi:10.1016/j.taap.2009.01.022

2007

  • Orr G., D.J. Panther, J.L. Phillips, B.J. Tarasevich, A. Dohnalkova, D. Hu, and J.G. Teeguarden, et al. 2007. "Submicron and Nanoscale Inorganic Particles Exploit the Actin Machinery to be Propelled Along Microvillilike Structures into Alveolar Cells." ACS Nano 1, no. 5:463-475. PNNL-SA-53625. doi:10.1021/nn700149r
  • Teeguarden J.G., P.M. Hinderliter, G. Orr, B.D. Thrall, and J.G. Pounds. 2007. "Particokinetics In Vitro: Dosimetry Considerations for In Vitro Nanoparticle Toxicity Assessments." Toxicological Sciences 95, no. 2:300-312. PNNL-SA-55055. doi:10.1093/toxsci/kfl165

2006

  • Oehmen C.S., T. Straatsma, G.A. Anderson, G. Orr, B.M. Webb-Robertson, R.C. Taylor, and R.W. Mooney, et al. 2006. "New Challenges Facing Integrative Biological Science in the Post-Genomic Era." Journal of Biological Systems 14, no. 2:275-293. PNNL-SA-45428.

2005

  • Dong J., L. Opresko, W.B. Chrisler, G. Orr, R.D. Quesenberry, D.A. Lauffenburger, and H.S. Wiley. 2005. "The Membrane-anchoring Domain of Epidermal Growth Factor Receptor Ligands Dictates Their Ability to Operate in Juxtacrine Mode." Molecular Biology of the Cell 16, no. 6:2984-2998. PNWD-SA-7019.
  • Hendriks B.S., G. Orr, A.H. Wells, H.S. Wiley, and D.A. Lauffenburger. 2005. "Parsing ERK Activation Reveals Quantitatively Equivalent Contributions From Epidermal Growth Factor Receptor and HER2 In Human Mammary Epithelial Cells." Journal of Biological Chemistry 280, no. 7:6157-6169. PNNL-SA-43533.
  • Orr G., D. Hu, S. Ozcelik, L. Opresko, H.S. Wiley, and S.D. Colson. 2005. "Cholesterol Dictates the Freedom of EGF Receptors and HER2 in the Plane of the Membrane." Biophysical Journal 89, no. 2:1362-1373. PNNL-SA-44634. doi:10.1529/biophysj.104.056192

2004

  • Harms G.S., G. Orr, and H.P. Lu. 2004. "Probing Ion Channel Conformational Dynamics Using Simultaneous Single-Molecule Ultrafast Spectroscopy and Patch-Champ Electric Recording." Applied Physics Letters 84, no. 10:1792-1794. PNNL-SA-40454.

2003

  • Harms G.S., G. Orr, and H.P. Lu. 2003. "Probing Single-Molecule Ion Channel Conformational Dynamics Using Fluorescence Imaging, Combined Ultrafast Spectroscopy, and Patch-Clamp Recording." Biophysical Journal 84, no. 2 Pt. 2 Supp.:123-A. PNNL-SA-41980.
  • Harms G.S., G. Orr, M. Montal, B.D. Thrall, S.D. Colson, and H.P. Lu. 2003. "Probing conformational changes of gramicidin ion channels by single-molecule patch-clamp fluorescence microscopy." Biophysical Journal 85, no. 3:1826-1838. PNNL-SA-37874.

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