METHODS AND SYSTEMS OF CHARACTERIZING AND COUNTING MICROBIOLOGICAL COLONIES
A means to rapidly count colonies of microorganisms on nutrient plates was developed. The practice may be used to speed many practices, procedures, and tests used in biology, microbiology, and medicine that rely in whole or in part on the ability to culture, enumerate, and assess microorganisms. The measurement tool, measurement process, and method of analysis will enable previously unattainable research into bacterial activity. The method is novel and valuable because it makes use of sensitive optical metrology to resolve growth of microorganisms much sooner than can be done with traditional methods. In short, white-light interferometry (WLI) is used to obtain high resolution 3-D profiles of microorganisms that have been placed on nutrient enriched agar plates. The resulting profiles have very fine sub-micron depth resolution and the ability to see single cells and microorganisms. Images may be captured at high magnification to observe a single microbe as it grows into a microcolony or images may be captured at low magnification to observe many microorganisms at once. Images are captured non-destructively which enables monitoring over time without apparent disruption microbial growth, activity, and processes. Traditional colony counting methods take 24 hrs for fast growing bacteria and up to 3 weeks for some species. This invention will allow researchers and clinicians to count colony forming units in a bacterial culture sample as early as 1-4 hrs after being applied to an agar plate. This invention would also require less material (smaller and fewer plates) and fewer resources (less incubation space and less time for incubation). A company that makes and sells WLI optical profilers would be able to modify their product hardware and software to accommodate this method. It could be sold as an add-on to their existing instrument. Traditionally, WLI instruments have been used to examine non-living hard surfaces (e.g., printed circuit boards, hard disks, machined parts, etc.). Licensing this technology would allow a maker of white-light interferometry instruments to expand into new markets, especially biomedical research and clinical testing. Users of this invention would benefit by performing colony counting much faster while using few resources and providing other relevant and significant morphological details that are not measurable by other means. This invention can be summarized as three major parts: A method to prepare, image, and analyze microbial cultures with WLI such that CFUs and other morphological traits can be rapidly assessed Culture plates with surface properties and optical characteristics that enhance and enable WLI imaging An integrated WLI imaging system for bacterial culture analysis that may include: modifications to existing WLI instruments or components such as sample holders or imaging stages, automated or semi-automated analysis routines implemented in software, and customized culture plates designed for WLI imaging. Full details of experiments done to demonstrate this invention are included in the appended manuscript.
Methods and Apparatuses for Cross-Ontologial Analytics
This invention disclosure presents a method and system that exploits the impact that Endocrine Disrupting Chemicals (EDCs) exert on gene products through receptor binding to refine the predictive power of Quantitative Structure-Activity Relationships (QSAR) modeling in identifying EDCs and to discover biomarkers associated with identified EDCs. The approach focuses on two main tasks: . Develop a framework to assess the toxicity of chemicals in terms of their impact at the gene/protein level . Use the impact of chemicals at the gene/protein level to identify biomarkers.
Dummy Record for tracking Patents licensed in from BYU in TMA 529150
Dummy invention report to obtain IPID for licensed in patents.
SYSTEMS AND METHODS FOR MONITORING ORGANISMS WITHIN AN AQUATIC ENVIRONMENT
Systems and methods for monitoring organisms within an aquatic environment are described. According to one aspect, an injectable acoustic transmission device includes a body configured to be injected inside of an organism, a transducer within the body and configured to convert a plurality of electrical signals into a plurality of data transmissions which are transmitted externally of the body and the organism, a plurality of circuit components within the body and configured to use electrical energy from a power source to generate the electrical signals which are provided to the transducer, and wherein the transducer defines an internal volume and at least one of the circuit components is provided within the internal volume of the transducer.
Assemblies, Riveted Assemblies, Methods for Affixing Substrates, and Methods for Mixing Materials to Form a Metallurgical Bond (iEdison No. 0685901-22-0263)
This invention is a process and tool for performing mechanical fastening of materials using magnesium rivets.
SELF-REPAIRING CEMENT POLYMER COMPOSITES AND PROCESSES OF MAKING AND USING SAME
New cement-polymer composites and processes of making and using are detailed. One exemplary cement-polymer composite include a Portland cement, an epoxide polymer, a thiol-containing crosslinking agent, and an optional phase separation inhibitor. These composites are dynamically self-healing, mechanically robust, and thermally stable in high temperature environments and can be expected to increase service lifetimes in various applications including energy producing wellbores.
DEVICES AND METHODS FOR PERFORMING SHEAR-ASSISTED EXTRUSION, EXTRUSION FEEDSTOCKS, EXTRUSION PROCESSES, AND METHODS FOR PREPARING METAL SHEETS
A process for forming extruded products using a device having a scroll face configured to apply a rotational shearing force and an axial extrusion force to the same preselected location on material wherein a combination of the rotational shearing force and the axial extrusion force upon the same location cause a portion of the material to plasticize, flow and recombine in desired configurations. This process provides for a significant number of advantages and industrial applications, including but not limited to extruding tubes used for vehicle components with 50 to 100 percent greater ductility and energy absorption over conventional extrusion technologies, while dramatically reducing manufacturing costs.
Aquatic Organism Tracking Devices, Systems and Associated Methods
Compared with MHK energy, widely-used hydropower have also been facing similar environmental concerns. To help investigate the potential of fish injury and mortality from passage through hydropower turbines, PNNL developed the JSATS. Recent JSATS development included several state-of-the-art acoustic transmitters, such as the injectable transmitter and the juvenile eel/lamprey transmitter. The latter is the world's smallest acoustic tag. Both these small transmitters have been successfully demonstrated in field studies and helped gather information on species of early life stages that had previously been unobtainable. The highly efficient transducer and circuit designs as well as the high-density micro-battery technology specifically developed for these transmitters were the innovations that made these technological advancements possible. The JSATS operates at 416.7 kHz, a relatively high acoustic frequency that works well filtering out acoustic noises in freshwater environments. With hardware and software modifications, these technologies can be readily adopted for a lower-frequency transmitter for use in marine environments. Our feasibility assessment and laboratory benchtop testing of the transmitter concept at three different frequencies around 200 kHz have shown significant improvements (detailed results listed in the attached document).