NANOSCALE BIOCHEMICAL SAMPLE PREPARATION AND ANALYSIS
Provided herein are methods and systems for biochemical analysis, including compositions and methods for processing and analysis of small cell populations and biological samples (e.g., a robotically controlled chip-based nanodroplet platform). In particular aspects, the methods described herein can reduce total processing volumes from conventional volumes to nanoliter volumes within a single reactor vessel (e.g., within a single droplet reactor) while minimizing losses, such as due to sample evaporation.
NUCLEAR REACTOR ASSEMBLIES, NUCLEAR REACTOR TARGET ASSEMBLIES, AND NUCLEAR REACTOR METHODS (iEdison No. 0685901-16-0012)
Reactor target assemblies are provided that can include a housing defining a perimeter of at least one volume and Np or Am spheres within the one volume. Reactor assemblies are provided that can include a reactor vessel and a bundle of target assemblies within the reactor vessel, at least one of the target assemblies comprising a housing defining a volume with Np or Am spheres being within the volume. Irradiation methods are also provided that can include irradiating Np or Am spheres within a nuclear reactor, then removing the irradiated spheres from the reactor and treating the irradiated spheres.
Method and Apparatus for Distributed Intrusion Protection System for Ultra High Bandwidth Networks
Our hypothesis will allow high-speed data networks to perform network protocol anomaly detection and countermeasures in real time. An Adaptive Hybrid Pervasive Defense Matrix (AHPDM) will provide an innovative architecture that yields N-scalable intrusion protection capability while minimizing or eliminating adverse impacts on traffic throughput. This proposed solution will also provide a foundation for real time anomaly detection. The rationale behind this method is that a significant performance improvement will be realized by dividing the incoming data stream into separate "channels" (implemented in differing wavelengths of light) and then performing a parallel processing function .for each protocol stream. We assert that this data segregation technique of channel separation greatly simplifies the process of protocol anomaly analysis required for a full spectrum protection environment. The idea is in the concept stage.
Method for Autonomous Establishment and Utilization of An Active-RF Tag Network
The Battelle-conceived "FlexiTag" is a small, bendable/flexible RF active sensor tag. The intent is for the tag to be in a self-contained, molded package (1.25" x 1.5" x 0.2") that could conform to objects that have rounded or curved surfaces. The package would be totally encased in a pliable package making it impervious t the Help environment such as moisture and dust. Due to the ultimate projected price being inexpensive, the tag will be a disposal device - no maintenance such as battery replacement. For ease of use the tag would have a peel-off adhesive backing for mounting.
FIREARM DISCHARGE LOCATION SYSTEMS AND ASSOCIATED METHODS (iEdison No. 0685901-19-0021)
Funds are required to develop, test and validate a low-cost portable, suitable for setup by two officers, system for detection, echo-location and weapon type classification of firearm discharges in outdoor and indoor environments. The resulting sensor suite will be suitable for securing a typical public event involving hundreds of people. This system will be comprised of modified hardware and software from PNNL's Acoustic Gunshot Detection Technology for confined spaces (USPTO 10089845B2), which is capable of detection and weapon classification, along with time-stamping, recording events and alerting. PNNL's acoustic gunshot detector for confined spaces has been licensed by three commercial entities, one of which already has demonstrated and deployed a full solution in schools across the US, which includes the complete response and communication framework necessary to inform law enforcement and first responders. See, for instance, the EAGL Gunshot Detection and Lockdown System. The development of the portable system will leverage these existing response and communication frameworks providing instantaneous information of a firearm discharge simultaneously to law enforcement and first responders. Appropriate communication methods and protocols will be built into the portable detection system to enable a 'plug and play" configurability of the flexible network of sensors. This prototype and development user interface software will also be suitable for further development and integration into additional commercial First Responder alert systems, which will include outputs for CAD and CAMEO systems as well as other parameters defined by commercial partner. Development will be performed in collaboration with the Kennewick Police Department as we collaborated with this force in development and testing of the Acoustic Gunshot Detection Technology mentioned above (Luzi, Gonzalez, et al. Acoustic firearm discharge detection and classification in an enclosed environment, Journal of the Acoustical Society of America 139(5) 2016, pg. 2723-2731.). During preliminary prototype testing all system components will be deployed and operated by members of the Kennewick Police Force and feedback on design incorporated into the final engineering prototype. An engineering prototype technology transfer package comprised of technical drawings, electronic circuit specifications, and analysis software source code will be produced along with validation data for a selected set of scenarios.
SYSTEM AND PROCESS FOR DISSOLUTION OF SOLIDS
A system and process are disclosed for dissolution of solids and “difficult-to-dissolve” solids. A solid sample may be ablated in an ablation device to generate nanoscale particles. Nanoparticles may then swept into a coupled plasma device operating at atmospheric pressure where the solid nanoparticles are atomized. The plasma exhaust may be delivered directly into an aqueous fluid to form a solution containing the atomized and dissolved solids. The composition of the resulting solution reflects the composition of the original solid sample.
COLLECTION, RELEASE, AND DETECTION OF ANALYTES WITH POLYMER COMPOSITE SAMPLING MATERIALS (iEdison No. 0685901-14-0005)
A unique fiber core sampler composition, related systems, and techniques for designing, making, and using the same are described. The sampler is used to interface with existing field instrumentation, such as Ion Mobility Spectrometer (IMS) equipment. Desired sampler characteristics include its: stiffness/flexibility; thermal mass and conductivity; specific heat; trace substance collection/release dependability, sensitivity and repeatability; thickness; reusability; durability; stability for thermal cleaning; and the like. In one form the sampler has a glass fiber core with a thickness less than 0.3 millimeter that is coated with a polymer including one or more of: polymeric organofluorine, polyimide, polyamide, PolyBenzlmidazole (PBI), PolyDiMethylSiloxane (PDMS), sulfonated tetrafluoroethylene (PFSA) and Poly(2,6-diphenyl-p-phenylene Oxide) (PPPO). Multiple polymer coatings with the same or different polymer types may be included, core/substrate surface functionalization utilized, and/or the core/substrate may be at partially filled with thermally conductive particles.
Circularly Polarized Antennas for Active Holographic Imaging through Barriers
Circular polarized antennas and balun structure are dielectrically loaded to optimally match the the dielectric barrier material but yet are still light-weight in design. The circular polarized antennas allow optimal imaging performance because they remove the barrier front surface reflections, optimally couple the electromagnetic energy into the barrier, and remove issues associated with polarized dependent objects embedded in the barrier structure.
SYSTEM AND PROCESS FOR PURIFICATION OF ASTATINE-211 FROM TARGET MATERIALS (iEdison No. 0685901-17-0014)
A new column-based purification system and approach are described for rapid separation and purification of the alpha-emitting therapeutic radioisotope .sup.211At from dissolved cyclotron targets that provide highly reproducible product results with excellent .sup.211At species distributions and high antibody labeling yields compared with prior art manual extraction results of the prior art that can be expected to enable enhanced production of purified .sup.211At isotope products suitable for therapeutic medical applications such as treatment of cancer in human patients.