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Worldwide Hydrobiogeochemistry Observation Network for Dynamic River Systems (WHONDRS)

  • Team
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    • 48-Hour Diel Cycling Study
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  1. Home
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  3. Worldwide Hydrobiogeochemistry Observation Network for Dynamic River Systems (WHONDRS)

Methods

Field Protocols

Below are links to written and video field protocols used in WHONDRS campaigns. If you have a WHONDRS kit, please be aware that protocols may change over time, and you should follow the protocol that was sent with your kit. We encourage you to use or modify WHONDRS protocols for your own research. If you do, please cite the protocol using the ESS-DIVE data package DOI for the specific study (listed below). If you have any questions or would like additional information, please reach out to us at whondrs@pnnl.gov.

Global Bio-Chemo-Geography Surface Water Mini-Kit Sampling Protocol (SFA)

File

Mini Kit Sampling Protocol

DOI: 10.15485/1484811

 

Perturbation Response Traits Sediment Sampling Protocol (ECA)

File

Sediment Sampling Protocol

DOI: 10.15485/1834208

 

Perturbation Response Traits iButton Deployment Protocol (ECA)

File

iButton Deployment Protocol

DOI: 10.15485/1834207

Summer 2019 Sampling Surface Water and Sediment Protocol (SFA)

File

Summer 2019 Sampling Surface Water and Sediment Protocol

DOI: 10.15485/1729719

 

48-Hour Diel Cycling Study Surface Water and Pore Water Sampling Protocol (SFA)

File

Diel Sampling Protocol

DOI: 10.15485/1577265

ICON-ModEx Sampling and Sensor Deployment Protocol (SFA)

File

ICON-ModEx-Field-Protocol.pdf

DOI: Not yet published on ESS-DIVE

Laboratory and Data Protocols

We will be posting laboratory and data protocols in the future. While this section is under development, please reach out to whondrs@pnnl.gov if you have questions about specific laboratory protocols. 

 

Instrumentation

Below is some of the key instrumentation used for WHONDRS data generation. The exact instrumentation depends on the specific study, and in some cases, a given parameter may be generated from different instruments in different studies. In published WHONDRS data packages, instrumentation and methods information can be found in the metadata subfolder. If you have any questions or would like additional information, please reach out to whondrs@pnnl.gov.

 

  • Ultra high resolution organic matter characterization/metabolomics via 12-Tesla Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (FTICR-MS)

        FTICR samples are acidified with phosphoric acid and undergo solid phase extraction (SPE) prior to analysis

  • Sediment elemental abundances via Bruker M4 Plus XRF 
  • Bulk C/N via  Elementar Carbon Analyzer
  • NPOC and TN via Shimadzu combustion carbon analyzer TOC-L CSH/CSN E100V with ASI-L auto sampler
  • Respiration via time series, non-invasive dissolved oxygen measurements using Fibox 3 PreSens GmbH (optical oxygen meter)
  • EEMs via Horiba Aqualog Extended-UV
  • Anions via a Dionex ICS-2000 anion chromatograph with AS40 auto sampler

        Beginning in 2019, samples are refrigerated and analyzed for anions within 30 days of collection following USGS guidance for NO3

  • Cations via Perkin Elmer Optima 2100 DV ICP-OES with an AS93 auto sampler

        Cations samples are acidified with nitric acid 


 

Projects tagged as SFA are part of the River Corridor Hydrobiogeochemistry Science Focus Area (SFA) project funded by the U.S. Department of Energy (DOE) Biological and Environmental Research (BER) Earth Systems Science (ESS) Program.

Projects tagged as ECA are part of the Perturbation Response Traits project funded by the U.S. Department of Energy's Early Career Research Program

Research topics

Subsurface Science
Earth & Coastal Sciences
Biology

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