PNNL

Abstract

Ketamine produces rapid antidepressant effects, yet its molecular interactions and mechanisms in human neurons remain poorly understood. Traditional tissue-based assays lack the spatial, temporal, and biological context needed to capture dynamic drug responses. This project has developed a framework for characterizing drug interactions using ketamine as a model by defining molecular targets in human iPSC-derived neurons, mapping multi-omics responses to physiologically relevant exposure, and applying chemical probes and multimodal imaging to visualize intracellular dynamics and identify non-canonical targets.