Photoswitchable fluorescent probes capable of the targeted labeling of tagged proteins are of significant interest due to their ability to enable in situ imaging of protein complexes within native biomolecular assemblies. Here we describe the synthesis of a fluorescent probe (AsCy3Cy5), and demonstrate the targeted labeling and super-resolution imaging of a tagged protein within a supramolecular protein complex.
Revised: August 15, 2014 |
Published: November 14, 2012
Citation
Fu N., Y. Xiong, and T.C. Squier. 2012.Synthesis of a Targeted Biarsenical Cy3-Cy5 Affinity Probe for Superresolution Fluorescence Imaging.Journal of the American Chemical Society 134, no. 45:18530-18533.PNNL-SA-90396.doi:10.1021/JA308503x