A set of 424 nonmembrane proteins from Methanobacterium thermoautotrophicum were cloned, expressed and purified for structural studies. Of these, ~20% were found to be suitable candidates for X-ray crystallographic or NMR spectroscopic analysis without further optimization of conditions, providing an estimate of the number of the most accessible structural targets in the proteome.
Revised: July 24, 2020 |
Published: October 1, 2000
Citation
Christendat D., A. Yee, A. Dharamsi, Y. Kluger, A. Savchenko, J.R. Cort, and V. Booth, et al. 2000.Structural Proteomics of an Archaeon.Nature Structural & Molecular Biology 7, no. 10:903-909.PNNL-SA-34008.doi:10.1038/82823