April 3, 2024
Journal Article

Spatial Proteomics toward Subcellular Resolution by Coupling Deep Ultraviolet Laser Ablation with Nanodroplet Sample Preparation

Abstract

Multiplexed molecular profiling of tissue microenvironments, or spatial omics, can provide critical insights into cellular functions and disease pathology. The coupling of laser microdissection with mass spectrometry-based proteomics has enabled deep and unbiased mapping of >1000 proteins. However, the throughput of laser microdissection is often limited due to tedious two-step procedures, sequential laser cutting, and sample collection. The two-step procedure also hinders the further improvement of spatial resolution to

Published: April 3, 2024

Citation

Xiang P., A.V. Liyu, Y. Kwon, D. Hu, S.M. Williams, D. Velickovic, and L. Markillie, et al. 2023. Spatial Proteomics toward Subcellular Resolution by Coupling Deep Ultraviolet Laser Ablation with Nanodroplet Sample Preparation. ACS Measurement Science Au 3, no. 6:459–468. PNNL-SA-191203. doi:10.1021/acsmeasuresciau.3c00033

Research topics

Spatial and Single-Cell Proteomics