The sandwich ELISA microarray is a powerful screening tool in biomarker discovery and validation due to its ability to simultaneously probe for multiple proteins in a miniaturized assay. The technical challenges of generating and processing the arrays are numerous. However, careful attention to possible pitfalls in the development of your antibody microarray assay can overcome these challenges. In this chapter, we describe in detail the steps that are involved in generating a reliable and reproducible sandwich ELISA microarray assay.
Revised: June 11, 2009 |
Published: May 11, 2009
Citation
Gonzalez R.M., S.M. Varnum, and R.C. Zangar. 2009.Sandwich ELISA Microarrays: Generating Reliable and Reproducible Assays for High-Throughput Screens. In Biomarker Methods in Drug Discovery and Development, edited by F Weng. 273-290. Totowa, New Jersey:Humana Press.PNNL-SA-53301.