We have synthesized a red biarsenical fluorescent probe, AsCy3, with good photostability, low pH sensitivity, high absorbance and good quantum yield. It is directed specifically to a small tetracysteine peptide binding motif Cy3TAG (CysCysLysAlaGluAlaAlaCysCys) in the presence of other tetracysteine tags. This new probe provides a FRET partner to biarsenical dye FlAsH, making this discovery an important step toward a whole toolkit of colored probes directed to different small peptide motifs.
Revised: July 31, 2007 |
Published: July 24, 2007
Citation
Cao H., Y. Xiong, T. Wang, B. Chen, T.C. Squier, and M.U. Mayer. 2007.A Red Cy3-Based Biarsenical Fluorescent Probe Targeted to a Complementary Binding Peptide.Journal of the American Chemical Society 129.PNNL-SA-55586.doi:10.1021/ja070003c