June 1, 2007
Book Chapter

A Protein Microarray ELISA for the Detection of Botulinum neurotoxin A

Abstract

An enzyme-linked immunosorbent assay (ELISA) microarray was developed for the specific and sensitive detection of botulinum neurotoxin A (BoNT/A), using high-affinity recombinant monoclonal antibodies against the receptor binding domain of the heavy chain of BoNT/A. The ELISA microarray assay, because of its sensitivity, offers a screening test with detection limits comparable to the mouse bioassay, with results available in hours instead of days.

Revised: February 22, 2011 | Published: June 1, 2007

Citation

Varnum S.M. 2007. A Protein Microarray ELISA for the Detection of Botulinum neurotoxin A. In Biodetection Technologies: Technological Responses to Biological Threats, 4th ed. Brookline, Massachusetts:Knowledge Press. PNNL-SA-56187.