A sensitive electrochemical immunosensor based on poly(guanine) functionalized silica nanoparticle label has been developed for the detection of protein biomarker, biomarker recombinant human tumor necrosis factor-a(TNF-a).This method is simple, selective, and reproducible for trace biomarker analysis. A remarkable LOD has been achieved through dual signal amplification by poly[G] functionalized silica NPs and catalytic guanine oxidation. The work demonstrates the feasibility of developing an inexpensive, sensitive, and portable device for multiplexed diagnoses of different protein biomarkers.
Revised: July 28, 2006 |
Published: June 1, 2006
Citation
Liu G., J. Wang, and Y. Lin. 2006.Nanoparticle Labels/ Electrochemical Immunosensor for Detection of Biomarkers. In Nanotech 2006 Technical Proceedings of the 2006 NSTI Nanotechnology Conference and Trade Show, edited by M. Lauden, R. Romanowicz, 2, 192-195. Cambridge, Massachusetts:Nano Science & Technology Institute.PNNL-SA-49687.