Characterizing protein-ligand binding dynamics is crucial for understanding protein function and developing new therapeutic agents. We have developed a novel microfluidic platform that features rapid mixing of protein and ligand solutions, variable incubation times, and on-chip electrospray ionization to perform label-free, solution-based monitoring of protein-ligand binding dynamics. This platform offers many advantages including automated processing, rapid mixing, and low sample consumption.
Revised: May 19, 2016 |
Published: March 24, 2016
Citation
Cong Y., S. Katipamula, C.D. Trader, D.J. Orton, T. Geng, E.S. Baker, and R.T. Kelly. 2016.Mass Spectrometry-Based Monitoring of Millisecond Protein-Ligand Binding Dynamics Using an Automated Microfluidic Platform.Lab on a Chip 16, no. 9:1544-1548.PNNL-SA-115886.doi:10.1039/c6lc00183a