Quantitative analysis of liquid chromatography (LC)- mass spectrometry (MS) and tandem mass spectrometry (MS/MS) data is essential to many proteomics studies. We have developed MASIC to accurately measure peptide abundances and LC elution times in low-resolution LC-MS/MS analyses. This software program uses an efficient processing algorithm to quickly generate mass specific selected ion chromatograms from a dataset and provides an interactive browser that allows users to examine individual chromatograms in a variety of fashions. The improved elution time estimates afforded by MASIC increase the utility of LC-MS/MS data in the accurate mass and time (AMT) tag approach to proteomics.
Revised: October 16, 2015 |
Published: June 1, 2008
Citation
Monroe M.E., J.L. Shaw, D.S. Daly, J.N. Adkins, and R.D. Smith. 2008.MASIC: a software program for fast quantitation and flexible visualization of chromatographic profiles from detected LC-MS(/MS) features.Computational Biology and Chemistry 32, no. 3:215-217.PNNL-SA-52621.doi:10.1016/j.compbiolchem.2008.02.006