August 20, 2025
Journal Article

Intestinal microbiome profile of the brown rock sea cucumber (Holothuria glaberrima) using ITS and 16S rDNA amplicons from direct mechanical, enzymatic, and chemical metagenomic extraction

Abstract

Using direct mechanical, enzymatic, and chemical extraction methods, the intestinal microbiome of the marine invertebrate Holothuria glaberrima was obtained. ITS and 16S rDNA regions were sequenced to enrich and investigate the prokaryotic and fungal diversity profiles from different anatomical regions within the sea cucumber’s intestinal biology.

Published: August 20, 2025

Citation

Nieves-Morales R., J.A. Paez-Diaz, S.M. Rodriguez-Carrio, G. Melendez-Martinez, E.O. Rivera-Lopez, J.A. Rodriguez-Ramos, and J. Garcia-Arraras, et al. 2025. Intestinal microbiome profile of the brown rock sea cucumber (Holothuria glaberrima) using ITS and 16S rDNA amplicons from direct mechanical, enzymatic, and chemical metagenomic extraction. Microbiology Resource Announcements 14, no. 7:e00293-25. PNNL-SA-215122. doi:10.1128/mra.00293-25

Research topics

Microbiome Science