It is demonstrated that a high resolution magic angle spinning 1H-NMR spectrum of biological tissue samples with volumes as small as 150 nanoliters, or 0.15 mg in weight, can be acquired in a few minutes at 21.1 T magnetic field using a commercial 1.6 mm fast-MAS probe with minor modification of the MAS rotor. The strategies of sealing the samples inside the MAS rotor to avoid fluid leakage as well as the ways of optimizing the signal to noise are discussed.
Revised: May 21, 2013 |
Published: March 5, 2013
Citation
Feng J., J.Z. Hu, S.D. Burton, and D.W. Hoyt. 2013.High Resolution Magic Angle Spinning 1H-NMR Metabolic Profiling of Nanoliter Biological Tissues at High Magnetic Field.Chinese Journal of Magnetic Resonance 30, no. 1:1-11.PNNL-SA-89007.