We describe a genetic system for producing specific gene knockouts in Cyanothece sp. Strain PCC 7822 using a ssDNA technique (37). The first fully segregated mutant was in the nifK gene (DnifK) and the mutant was unable to grow on a medium lacking combined nitrogen and produced virtually no hydrogen.
Revised: October 31, 2011 |
Published: November 1, 2010
Citation
Min H., and L.A. Sherman. 2010.Genetic Transformation and Mutagenesis Via Single-Stranded DNA in the Unicellular, Diazotrophic Cyanobacteria of the Genus Cyanothece.Applied and Environmental Microbiology 76, no. 22:7641-7645. doi:10.1128/AEM.01456-10