Expression of Trichoderma reesei exo-cellobiohydrolase I (CBHI) gene in transgenic tobacco was under the control of CaMV 35S promoter. In transgenic leaf tissues, CBHI activity up to 66.1 mmol h^-1 g^-1 total protein was observed. In transgenic calli, the highest CBHI activity was 83.6 umol h^-1 g^-1 total protein. Protein immunoblot analysis confirms the presence of CBHI enzyme in both transgenic calli and leaf tissues. CBHI expression levels accounted for about 0.11% and 0.082% of total protein in transgenic leaf tissues and calli, respectively. Furthermore, expression of CBHI gene did not affect normal growth and development of transgenic plants.
Revised: July 18, 2002 |
Published: March 1, 1999
Citation
Dai Z., B.S. Hooker, R.D. Quesenberry, and J. Gao. 1999.Expression of Trichoderma reesei exo-cellobiohydrolase I transgenic tobacco leaves and calli.Applied Biochemistry and Biotechnology 77, no. 9:689-699.PNNL-SA-31600.