Evaluation of a High Intensity Focused Ultrasound-Immobilized Trypsin Digestion and 18 O-Labeling Method for Quantitative Proteomics

August 1, 2009

Journal Article

Evaluation of a High Intensity Focused Ultrasound-Immobilized Trypsin Digestion and 18 O-Labeling Method for Quantitative Proteomics

Abstract

A new method that uses immobilized trypsin concomitant with ultrasonic irradiation results in ultra-rapid digestion and thorough 18O labeling for quantitative protein comparisons. The reproducible and highly efficient method provided effective digestions in

Revised: September 8, 2010 | Published: August 1, 2009

Citation

Lopez-Ferrer D., K.K. Hixson, H.S. Smallwood, T.C. Squier, K. Petritis, and R.D. Smith. 2009. Evaluation of a High Intensity Focused Ultrasound-Immobilized Trypsin Digestion and 18 O-Labeling Method for Quantitative Proteomics. Analytical Chemistry 81, no. 15:6272-6277. PNNL-SA-63667.