We developed an ELISA in high-density microassay format to detect hepatocyte growth factor (HGF) in human serum. The microassay can detect HGF at sub-pg/mL concentrations in sample volumes of 100 uL or less. The microassay is also quantitative and was used to detect elevated HGF levels in sera from recurrent breast cancer patients. The microarry format provides the potential for high-throughput quantitation of multiple biomarkers in parallel.
Revised: September 11, 2002 |
Published: June 3, 2002
Citation
Woodbury R.L., S.M. Varnum, and R.C. Zangar. 2002.Elevated HGF Levels in Sera from Breast Cancer Patients Detected Using a Protein Microarray ELISA.Journal of Proteome Research 2002, no. 1:233-237. PNWD-SA-5644.