In the past decade, many new strategies for mass spectrometry (MS)-based analyses of lipids have been developed. Lipidomics is one of the most promising research fields to emerge as a result of these advances in MS. Currently, mass spectrometric analysis of lipids involves two complementary approaches: direct infusion (shotgun lipidomics) and liquid chromatography coupled to MS. In this chapter, I will demonstrate the approach of shotgun lipidomics using electrospray ionization tandem MS for the analysis of lipid molecular species directly from crude biological extracts of tissue or fluids.
Revised: May 19, 2011 |
Published: January 11, 2011
Citation
Mezengie G.I. 2011.Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS)-Based Shotgun Lipidomics. In Metabolic Profiling: Methods and Protocols. Methods in Molecular Biology, edited by TO Metz. 259-275. New York:Humana Press.PNNL-SA-71378.