ABSTRACT-We report on the development and validation of simple microarray method for the direct detection of intact 16S rRNA from un-purified soil extracts. Total RNA from Geobacter chapellei and Desulfovibrio desulfuricans was hybridized to an oligonculeotide array consisting of universal and species-specific 16S rRNA probes...
Revised: October 27, 2005 |
Published: October 1, 2001
Citation
Small J.A., D.R. Call, F.J. Brockman, T.M. Straub, and D.P. Chandler. 2001.Direct Detection of 16S rRNA in Soil Extracts by Using Oligonucleotide Microarrays.Applied and Environmental Microbiology 67, no. 10:4708-4716.PNNL-SA-35125.