In this paper, we describe the design and fabrication process of a polydimethylsiloxane (PDMS) microchip for on-chip multiplex immunoassay application. The microchip consists of a PDMS microfluidic channel layer and a micro pneumatic valve control layer. By selectively pressurizing the pneumatic microvalves, immuno reagents were controlled to flow and react in certain fluidic channel sites. Cross contamination was prevented by tightly closed valves. Our design was proposed to utilize PDMS micro channel surface as the solid phase immunoassay substrate and simultaneously detect four targets antigens on chip. Experiment result shows that 20psi valve pressure is sufficient to tightly close a 200µm wide micro channel with flow rate up to 20µl/min.
Revised: December 1, 2010 |
Published: July 1, 2010
Citation
Shao G., W. Wang, J. Wang, and Y. Lin. 2010.Design and Fabrication of a PDMS Microchip Based Immunoassay. In Microfluidics, BioMEMS, and Medical Microsystems VIII: Proceedings of the SPIE, edited by H Becker and W Wang, 7593, Paper No. 75930Q. Bellingham, Washington:SPIE.PNNL-SA-73305.doi:10.1117/12.847138