ON-DEMAND SERIAL DILUTION USING QUANTIZED NANO/PICOLITER-SCALE DROPLETS

October 29, 2014

Conference Paper

ON-DEMAND SERIAL DILUTION USING QUANTIZED NANO/PICOLITER-SCALE DROPLETS

Abstract

This paper describes a fully automated droplet-based microfluidic device for on-demand serial dilution that is capable of achieving a dilution ratio of >6000 (concentration ranges from 1 mM to 160nM) over 35 nanoliter-scale droplets. This serial diluter can be applied to high throughput and label-free kinetic assays by integrating with our previously developed on-demand droplet-based microfluidic with mass spectrometry detection.

Revised: March 3, 2015 | Published: October 29, 2014

Citation

Jambovane S.R., S.A. Prost, A.M. Sheen, J.K. Magnuson, and R.T. Kelly. 2014. ON-DEMAND SERIAL DILUTION USING QUANTIZED NANO/PICOLITER-SCALE DROPLETS. In Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014), October 26-30, 2014, San Antonio, Texas, 1247-1249. San Diego, California:Chemical and Biological Microsystems Society. PNNL-SA-107700.