A biarsenical fluorescent probe 5-carboxy-4’,5’-bis(1,3,2-dithioarsolan-2-yl)fluorescein (CrAsH) was conveniently synthesized from 5-carboxyfluorescein. This probe shows highly selective binding to tetracysteine tags and low hydrophobic non-specific binding in vitro. It provides a potential application for protein labeling and cell imaging.
Revised: June 28, 2006 |
Published: May 18, 2006
Citation
Cao H., B. Chen, T.C. Squier, and M.U. Mayer. 2006.CrAsH: A Biarsenical Multi-use Affinity Probe with low non-specific fluorescence.Chemical Communications.PNNL-SA-48401.doi:10.1039/b602699k