Cellulases and other enzymes are needed for saccharification of plant biomass in the biorefinery industry. Expression, characterization, and eventual large-scale production of known and novel cellulases requires the ability to express and secrete heterologous enzymes in relevant protein production platforms like Aspergillus niger. A method for cloning and expression of genes for these desirable enzymes in A. niger is presented in this Chapter.
Revised: March 1, 2021 |
Published: June 1, 2018
Citation
Reilly M.C., S.A. Campen, B.A. Simmons, S.E. Baker, J.M. Gladden, and J.K. Magnuson. 2018.Cloning and Expression of Heterologous Cellulases and Enzymes in Aspergillus niger. In Cellulases: Methods and Protocols. Methods in Molecular Biology, edited by M Lubeck. 123-133. New York, New York:Humana Press.PNNL-SA-135995.doi:10.1007/978-1-4939-7877-9_10