Characterization of prokaryotic communities in Puerto Rican caves using 16S rDNA amplicon sequencing

August 26, 2025

Journal Article

Characterization of prokaryotic communities in Puerto Rican caves using 16S rDNA amplicon sequencing

Abstract

The cave ecosystems host microbial communities adapted to extreme environments. This study utilized 16S rDNA to investigate the prokaryotic diversity across seven caves in Puerto Rico’s northern limestone karst belt. Microbial profilingrevealed distinct subterranean communities, enhancing our understanding of cave microbiology and potential applications in environmental conservation and microbial research.

Published: August 26, 2025

Citation

Perez-Santos N., S. Borrero-Villabol, R. Nieves-Morales, J.A. Paez-Diaz, E.O. Rivera-Lopez, J.A. Rodriguez-Ramos, and A.M. Nieves-Rivera, et al. 2025. Characterization of prokaryotic communities in Puerto Rican caves using 16S rDNA amplicon sequencing. Microbiology Resource Announcements 14, no. 7:e00354-25. PNNL-SA-215178. doi:10.1128/mra.00354-25

Research topics

Microbiome Science

PNNL

Characterization of prokaryotic communities in Puerto Rican caves using 16S rDNA amplicon sequencing

Abstract

Citation

Research topics

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Intestinal microbiome profile of the brown rock sea cucumber (Holothuria glaberrima) using ITS and 16S rDNA amplicons from direct mechanical, enzymatic, and chemical metagenomic extraction

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