We describe methods for broad characterization of the human plasma proteome. The combination of stepwise IgG and albumin protein depletion by affinity chromatography and ultrahigh-efficiency capillary liquid chromatography separations coupled to ion trap-tandem mass spectrometry enabled identification of 2392 proteins from a single plasma sample with an estimated confidence level of >94%, and an additional 2198 proteins with an estimated confidence level of 80%. The relative abundances of the identified proteins span a range of over eight orders of magnitude in concentration (
Revised: November 29, 2005 |
Published: October 15, 2005
Citation
Shen Y., J. Kim, E.F. Strittmatter, J.M. Jacobs, D.G. Camp, R. Fang, and N. Tolic, et al. 2005.Characterization of the human blood plasma proteome.Proteomics 5, no. 15:4034-4045. PNWD-SA-6708.