Application of the 5' Fluorogenic Exonuclease Assay (TaqMan) for Quantitative rDNA and rRNA Analysis in Sediments.

June 1, 2001

Journal Article

Application of the 5' Fluorogenic Exonuclease Assay (TaqMan) for Quantitative rDNA and rRNA Analysis in Sediments.

Abstract

In this study, we report on the development of quantitative PCR and RT-PCR assays for the 16S rRNA of Geobacter sp., and identify key issues related to fluorogenic reporter systems for nucleic acid analyses in sediments.

Revised: June 28, 2001 | Published: June 1, 2001

Citation

Stults J.R., O. Snoeyenbos-West, B. Methe, D.R. Lovley, and D.P. Chandler. 2001. Application of the 5' Fluorogenic Exonuclease Assay (TaqMan) for Quantitative rDNA and rRNA Analysis in Sediments. Applied and Environmental Microbiology 67, no. 6:2781-2789. PNNL-SA-34234.