Liquid chromatography coupled with mass spectrometry is the predominant platform used to analyze proteomics samples consisting of large numbers of proteins and their proteolytic products (e.g., truncated polypeptides) and spanning a wide range of relative concentrations. This review provides an overview of advanced capillary liquid chromatography techniques and methodologies that greatly improve separation resolving power and proteomics analysis coverage, sensitivity, and throughput.
Revised: December 18, 2012 |
Published: October 26, 2012
Citation
Xie F., R.D. Smith, and Y. Shen. 2012.Advanced proteomic liquid chromatography.Journal of Chromatography A 1261.PNNL-SA-86948.doi:10.1016/j.chroma.2012.06.098