PNNL

Angie Melville is a chemist with the Chemical and Biological Signatures Group within the National Security Directorate. Since joining the lab in 2000, she has made notable contributions across various research domains. Her work has been instrumental in characterizing Hanford tank waste and developing innovative analytical methods for biological agents and toxins. Melville has also enhanced the lab's capabilities in detecting and analyzing hazardous substances. Additionally, she was part of the COVID-19 response team. Her expertise is further demonstrated by her research on fentanyl and other controlled substances, which has aided in their detection and in understanding their impact. Most recently, she has focused on addressing the critical environmental and safety concerns related to Hanford tank farm vapors.

BS in Physical Sciences, Washington State University

• Member of the American Chemical Society 

• Six Outstanding Performance Awards
• 2021 Laboratory Director’s Award for Operation Excellence, Institutional Achievement in Management and Operations for Covid Response Team
• 2022 U.S. Department of Energy Secretary of Energy Achievement Award

Leiser O.P., D.L. Auberry, E. Bakker, W.B. Chrisler, K.M. Engbrecht, H.E. Engelmann, and S.J. Fansler, et al. 2023. "Insights from a workplace SARS-CoV-2 specimen collection program, with genomes placed into global sequence phylogeny." PLoS One 18, no. 4:Art. No. e0285042. PNNL-SA-179220. doi:10.1371/journal.pone.0285042

Mobberley J.M., S.K. Cooley, M.W. Widder, S. Phillips, A.M. Melville, L.M. Brennan, and V.T. Divito, et al. 2022. "A multistep enrichment process with custom growth medium improves resuscitation of chlorine-stressed coliforms from secondary sewage effluents." Journal of Microbiological Methods 192. PNNL-SA-154032. doi:10.1016/j.mimet.2021.106364

Nims M.K., A.M. Melville, J.J. Moran, K.H. Jarman, and B.W. Wright. 2022. "Compound Specific Stable Isotope Analysis of Aromatics in Diesel Fuel to Identify Potential Cocktailing." Forensic Science International 334. PNNL-SA-149150. doi:10.1016/j.forsciint.2022.111244

Wojcik R., T.L. Oxford, A.M. Melville, C.W. Wright, and B.W. Wright. 2021. "Mapping glyceride species in biodiesel by high-temperature gas chromatography combined with chemical ionization mass spectrometry." Analytical Chemistry 93, no. 43:14432–14439. PNNL-SA-167048. doi:10.1021/acs.analchem.1c02474

Heller N.C., A.M. Garrett, E.D. Merkley, S. Cendrowski, A.M. Melville, J.S. Arce, and S.C. Jenson, et al. 2019. "Probabilistic limit of detection for ricin identification using a shotgun proteomics assay." Analytical Chemistry 91, no. 19:12399-12406. PNNL-SA-144376. doi:10.1021/acs.analchem.9b02721

Merkley E.D., S.C. Jenson, J.S. Arce, A.M. Melville, O.P. Leiser, D.S. Wunschel, and K.L. Wahl. 2017. "Ricin-Like Proteins from the Castor Plant Do Not Influence Liquid Chromatography-Mass Spectrometry Detection of Ricin in Forensically Relevant Samples." Toxicon 140. PNNL-SA-124754. doi:10.1016/j.toxicon.2017.10.004

Fraga C.G., A.V. Mitroshkov, N.S. Mirjankar, B.P. Dockendorff, and A.M. Melville. 2017. "Elemental Source Attribution Signatures for Calcium Ammonium Nitrate (CAN) Fertilizers used in Homemade Explosives." Talanta 174. PNNL-SA-123773. doi:10.1016/j.talanta.2017.05.066

Bartholomew R.A., R.M. Ozanich, J.S. Arce, H.E. Engelmann, A. Heredia-Langner, B.A. Hofstad, and J.R. Hutchison, et al. 2017. "Evaluation of Immunoassays and General Biological Indicator Tests for Field Screening of Bacillus anthracis and Ricin." Health Security 15, no. 1:81-96. PNNL-SA-116267. doi:10.1089/hs.2016.0044

Leiser OP, ED Merkley, BH Clowers, BLD Kaiser, A Lin, JR Hutchison, AM Melville, DM Wagner, PS Keim, J Foster, and HW Kreuzer. 2015. "Investigation of Yersinia pestis Laboratory Adaptation through a Combined Genomics and Proteomics Approach." PLoS One 10(11):Article No. e0142997. doi: 10.1371/journal.pone.0142997

Ehrhardt C, AM Melville, BH Clowers, JM Goss, DS Wunschel, OP Leiser, PS Keim, J Foster, and HW Kreuzer. 2013. " Carbohydrate Profiling and Multivariate Analysis of Serially Passaged Yersinia pestis Cultures Derived from a Natural Environment." PNNL-SA-97448, Pacific Northwest National Laboratory, Richland, WA. [Unpublished] 

Wunschel DS, AM Melville, CJ Ehrhardt, HA Colburn, KD Victry, KC Antolick, JH Wahl, and KL Wahl. 2012. "Integration of Gas Chromatography Mass Spectrometry Methods for Differentiating Ricin Preparation Methods." The Analyst 137(9):2077-2085. 

Wunschel DS, KL Wahl, AM Melville, CM Sorensen, HA Colburn, NB Valentine, and CL Stamper. 2011. "Determination of Post-Culture Processing with Carbohydrates by MALDI-MS and TMS derivatization GC/MS." Talanta 85(5):2352-2360. doi:10.1016/j.talanta.2011.07.073 

Colburn HA, DS Wunschel, KC Antolick, AM Melville, and NB Valentine. 2011. "The Effect of Growth Medium on B. anthracis Sterne Spore Carbohydrate Content." Journal of Microbiological Methods 85(3):183-189. doi:10.1016/j.mimet.2011.02.015

Colburn HA, DS Wunschel, HW Kreuzer-Martin, JJ Moran, KC Antolick, and AM Melville. 2010. "Analysis of Carbohydrate and Fatty Acid Marker Abundance in Ricin Toxin Preparations for Forensic Information." Analytical Chemistry 82(14):6040-6047.