METHODS AND SYSTEMS OF PROTEOME ANALYSIS AND IMAGING (NIH iEdison No. 0685901-18-0003)
Provided herein are methods and systems for proteome analysis that are at least partially automated and/or performed robotically. In some aspects, the methods and systems described herein can rapidly and efficiently provide protein identification of each of the proteins from a proteome, or a complement of proteins, obtained from extremely small amounts of biological samples. The identified proteins can be imaged quantitatively over a spatial region. Automation and robotics facilitates the throughput of the methods and systems, which enables protein imaging and/or rapid proteome analysis.
Methods and Systems for Detecting Abnormal Digital Traffic
Currently, the PNNL network infrastructure is monitored on 5X24 bases, with limited on-call response by cyber security staff to combat intrusion, policy infraction, or attempted access. Availability of cyber security staff is currently on a volunteer basis. Monitoring by operator staff is only Monday through Friday on a 24 hour basis. Existing security alerting systems are not autocratically linked to our perimeter protection, nor do we have clearly delineated processes in place to respond to critical alert during non-normal working hours. The reply to the aforementioned issues was to develop and implement an automated monitoring and response system that integrates our real-time alerting systems with automated controls to react ~o critical alerts. For example, when our real-time alerting systems identify a workstation scanning I outside networks; the firewall can automatically block that specific workstation IP address. Security staff would verify and resolve this critical alarm during normal operation hours.
Orthogonal Ion Injection Apparatus and Process
An orthogonal ion injection apparatus and process are described in which ions are directly injected into an ion guide orthogonal to the ion guide axis through an inlet opening located on a side of the ion guide. The end of the heated capillary is placed inside the ion guide such that the ions are directly injected into DC and RF fields inside the ion guide, which efficiently confines ions inside the ion guide. Liquid droplets created by the ionization source that are carried through the capillary into the ion guide are removed from the ion guide by a strong directional gas flow through an inlet opening on the opposite side of the ion guide. Strong DC and RF fields divert ions into the ion guide. In-guide orthogonal injection yields a noise level that is a factor of 1.5 to 2 lower than conventional inline injection known in the art. Signal intensities for low m/z ions are greater compared to convention inline injection under the same processing conditions..
NANOSCALE BIOCHEMICAL SAMPLE PREPARATION AND ANALYSIS
Provided herein are methods and systems for biochemical analysis, including compositions and methods for processing and analysis of small cell populations and biological samples (e.g., a robotically controlled chip-based nanodroplet platform). In particular aspects, the methods described herein can reduce total processing volumes from conventional volumes to nanoliter volumes within a single reactor vessel (e.g., within a single droplet reactor) while minimizing losses, such as due to sample evaporation.
BIOSEQUENCE-BASED APPROACH TO ANALYZING BINARIES
In a dynamic computing environment, it is a nontrivial task to verify code running in the environment because most approaches to software similarity require extensive and time-consuming analysis of a binary, or the approaches fail to recognize executables that are similar but nonidentical. A biosequence-based method for quantifying similarity of executable binaries is used to identify allowed codes in a real-world multi-user environment.
Information Processing Systems, Reasoning Modules, And Reasoning System Design Methods
Information processing systems, reasoning modules, and reasoning system design methods are described. According to one aspect, an information processing system includes working memory comprising a semantic graph which comprises a plurality of abstractions, wherein the abstractions individually include an individual which is defined according to an ontology and a reasoning system comprising a plurality of reasoning modules which are configured to process different abstractions of the semantic graph, wherein a first of the reasoning modules is configured to process a plurality of abstractions which include individuals of a first classification type of the ontology and a second of the reasoning modules is configured to process a plurality of abstractions which include individuals of a second classification type of the ontology, wherein the first and second classification types are different.
COMPLEMENTARY WORKFLOWS FOR IDENTIFYING ONE-HOP NETWORK BEHAVIOR AND MULTI-HOP NETWORK DEPENDENCIES
Enterprise networks are large, complex, and dynamic networks that must simultaneously satisfy an often disparate set of objectives. Comprising of multitude of network services and applications, understanding the functional relationships—a superset of dependencies--between components is paramount in support of business and missions. Understanding these relationships directly bolsters network management, fault detection and localization, and proactive and resilient cybersecurity defenses. The overview of the invention is as follows: starting from network flow information (NetFlow, IPFIX, etc.), a network flow information record is transformed, providing user-defined labels to the addresses and network engineering labels to other elements of the record. A tuple is created comprising elements of the record information, along with the transformed information. In the proof of concept, we define the tuple as (local network label, remote network label, remote network (IP) address, protocol, protocol information). Depending on the observation point of the flow sensor, the tuple can be expanded to included application, e.g, ('outlook", local network label, remote network label, remote network (IP) address, protocol, protocol information). An association rule algorithms—in our case, reinforced executions of frequent pattern (FP) growth—discovers relations between the elements of all the tuples. In simulated network testing, these relationships have characterized host-based network and application dependencies.
FOOTWEAR SCANNING SYSTEMS AND METHODS
Methods and apparatus for scanning articles, such as footwear, to provide information regarding the contents of the articles are described. According to one aspect, a footwear scanning system includes a platform configured to contact footwear to be scanned, an antenna array configured to transmit electromagnetic waves through the platform into the footwear and to receive electromagnetic waves from the footwear and the platform, a transceiver coupled with antennas of the antenna array and configured to apply electrical signals to at least one of the antennas to generate the transmitted electromagnetic waves and to receive electrical signals from at least another of the antennas corresponding to the electromagnetic waves received by the others of the antennas, and processing circuitry configured to process the received electrical signals from the transceiver to provide information regarding contents within the footwear.
GAMMA RADIATION STAND-OFF DETECTION, TAMPER DETECTION, AND AUTHENTICATION VIA RESONANT META-MATERIAL STRUCTURES
Resonant meta-material structures are defined by metallic, dielectric or other materials that form nanoshells or nanomeshes that can be situated proximate to ionizing-radiation-sensitive layers so as to provide ionizing-radiation-dose-dependent optical properties. Such meta-material structures can also define aligned or periodic, semi-random, or other arrangements of nanostructures that are coupled to or include stressed layers. Detection of optical radiation from such structures is used to determine gamma radiation dose or to detect a disturbance of the nanostructure indicating tampering.
DEVICE AND METHOD TO MANIPULATE IONS IN MULTI-LEVEL SYSTEM (NIH iEdison 0685901-19-0001, Grant No. CA217699)
This invention relates to increasing the ion path length in devices of planar geometry. In these devices, ions are usually manipulated between two of nearly parallel surfaces where the electric field is applied to each surface. The ion path length can be increased by moving ions from the first plane, where the first pair of surfaces manipulate ions, unto a second plane where the second pair of surfaces manipulate surfaces. Ions are urged to move from the first level of ion manipulation to the second level of ion manipulation through a transfer region the contains an orifice through which ions move. The orifice is located orthogonal to the first ion motion. Ions turn from the first direction into the orifice by applying one or more moving or static electric fields that cause ions to move from their primary path in the first level into the orifice and into the second level. A device that contains many levels (more than or equal 2 levels) can be then constructed. We also disclose a method to construct such a device to avoid ion losses and to simplify the assembly of such devices.