Holographic Imaging Based on Time-Domain Data of Natural-Fiber Containing Materials
Microwave and millimeter-wave holographic imaging has been well developed at PNNL [1-12]. Near real time imaging systems using this technology have been developed using linear arrays of microwave/millimeter wave antennas that are sequentially switched electronically to allow high-speed sampling along the array axis. Mechanical scanning in a perpendicular direction to the array axis then completes the sampling of a two dimensional aperture of wideband holographic image data. This data can then be reconstructed using the wideband holographic imaging algorithm resulting in a focused image. The wideband holographic imaging technique is described in detail in [4]. A similar scanning technique can be employed in a cylindrical fashion using a linear array that is scanned over a circular path around the target to be imaged [3, 5, 7]. The invention described in this report, is concerned with a specific technique used to spatially scan, or sample, along the axis of the array that results in a reduction of the number of physical antenna elements needed by approximately one-half compared to established techniques.
Low Pressure Electrospray Ionization System and Process for Effective Transmission of Ions
Achieving high sensitivity in electrospray ionization mass spectrometry (ESI-MS) is the key to effective analysis of complex biological sample. Every significant improvement in ESI-MS detection limit will enable applications otherwise impractical. Advances in ESI-MS sensitivity can also increase the dynamic range over which quantitative measurements can be performed. Currently, most sensitivity loss in ESI-MS is in the atmospheric pressure ESI interface region. The ion transmission through this interface is essentially limited by the small MS sampling inlet (typically 400 to 500 ƒÝm in diameter as required to maintain a good vacuum pressure in MS analyzer chamber) resulting in a
Ion manipulation device (iEdison NIH 0685901-13-0004.)
An ion manipulation method and device is disclosed. The device includes a pair of substantially parallel surfaces. An array of inner electrodes is contained within, and extends substantially along the length of, each parallel surface. The device includes a first outer array of electrodes and a second outer array of electrodes. Each outer array of electrodes is positioned on either side of the inner electrodes, and is contained within and extends substantially along the length of each parallel surface. A DC voltage is applied to the first and second outer array of electrodes. A RF voltage, with a superimposed electric field, is applied to the inner electrodes by applying the DC voltages to each electrode. Ions either move between the parallel surfaces within an ion confinement area or along paths in the direction of the electric field, or can be trapped in the ion confinement area.
Pressure Sensor Using Gas/Liquid Interface
The device is a MEMS based pressure sensor that uses a gas/liquid interface to transduce pressure. Simply put, liquid is moved into and out of a reservoir based on the external pressure. This movement is measured and directly correlates to the external pressure using gas laws and meniscus properties. There are three points of novelty. The first is the use of a single-port priming technique. The second is a meniscus auto-calibration, and the third is the use of electronic measuments. The single-port priming means that the device has only one inlet. It can be primed by placing it in a chamber at the 'priming' pressure (defined by the user). The chip is then submersed in the functional liquid and pressure is returned to atmosphere. The second is a meniscus autocalibration. In small channels, liquid/gas interfaces result in meniscus that have a significant pressure drop. By explointing geometric properties, this pressure drop can be directly measured and factored out of the final presure measurement with disregard for the environment, temperature, liquids, solids, and gasses. The third item is the conversion mechansim. All previously reported iterations of this concept use optical reporting. Including electrodes allows for capactive, tranductive, resistive, and direct digital electronic interrogation.
METHODS AND SYSTEMS OF PROTEOME ANALYSIS AND IMAGING (NIH iEdison No. 0685901-18-0003)
Provided herein are methods and systems for proteome analysis that are at least partially automated and/or performed robotically. In some aspects, the methods and systems described herein can rapidly and efficiently provide protein identification of each of the proteins from a proteome, or a complement of proteins, obtained from extremely small amounts of biological samples. The identified proteins can be imaged quantitatively over a spatial region. Automation and robotics facilitates the throughput of the methods and systems, which enables protein imaging and/or rapid proteome analysis.