PNNL

Abstract

This review will focus on some of the novel attributes of the yeast surface display platform for the discovery and characterization of novel affinity reagents, optimization of those reagents, and novel uses of the platform. This is not intended to serve as an exhaustive review on the broader topic of general scFv technologies (see (Winter et al., 1994; Smith and Petrenko, 1997; Bradbury et al., 2003) Furthermore, the scFv format of antibodies are easily manipulated through molecular cloning into a number of other formats such IgG, Fab, diabodies and such, for use in down steam applications and the reader is encouraged to read “IgG”, “Fab”, or your favorite format whenever scFv is seen in this review. This review is presented in 5 parts; 1) description of yeast display and its components, 2) library types and construction methods, 3) screening approaches for non-immune libraries and benefits, 4) screening approaches for directed evolution, kinetic on and off rates and 5) epitope complementation binning of clones.