The ribosomal protein S28E from the archaeon Methanobacterium thermoautotrophicum is a component of the 30S ribosomal subunit. Sequence homologs of S28E are found only in archaea and eukaryotes. Here we report the three-dimensional solution structure of S28E by NMR spectroscopy. S28E contains a globular region and a long C-terminal tail protruding from the core. The globular region consists of four antiparallel ?-strands which are arranged in a Greek-key topology. Unique features of S28E include an extended loop L2 that folds back onto the protein and a 12-residue charged C-terminal tail with no regular secondary structure and greater flexibility relative to the rest of the protein.
Revised: November 10, 2005 |
Published: December 1, 2003
Citation
Wu B., A. Yee, A. Pineda-Lucena, A. Semesi, T.A. Ramelot, J.R. Cort, and J. Jung, et al. 2003.Solution Structure of Ribosomal Protein S28E From Methanobacterium Thermoautotrophicum.Protein Science 12, no. 12:2831-2837. PNWD-SA-6105.