Automated microfluidic analysis has historically been carried out by flow injection analysis techniques. Sequential injection analysis represents a more versatile method for automated fluid handling. We have explored the use of sequential injection analysis for performing microcolumn separations. These separations can be used as part of a microanalytical procedure, or for sample preparation. In addition, with detection of retained species on the microcolumn, sequential injection separation represents a technique for sensing. Recently, it has been demonstrated that sequential injection separation can be carried out with renewable separation columns- the beads with interactive surfaces can be delivered to the microcolumn, used for processing the sample, and discard after each measurement. Delivery of new beads for each measurement analysit and bioanalytical chemistry will be presented.
Revised: September 24, 2002 |
Published: October 1, 1999
Citation
Grate J.W., O.B. Egorov, and C.J. Bruckner-Lea. 1999.Sequential Injection Separation and Sensing. In Chemical Microsensors and Applications II, Proceedings of SPIE, edited by S. Buttgenbach, 3857, 70-75. Bellingham, Washington:SPIE-The International Society for Optical Engineering.PNNL-SA-30966.