A new sample processing workflow that uses high intensity focused ultrasound to rapidly reduce and alkylate cysteines, digest proteins and then label peptides with 18O was developed for quantitative proteomics applications. Each step was individually refined to minimize reaction times, peptide loses and undesired by-products or modifications. By using this novel workflow, mouse plasma proteins were successfully denatured, alkylated, in-solution digested, and 18O labelled in
Revised: October 14, 2015 |
Published: September 1, 2008
Citation
Lopez-Ferrer D., T.H. Heibeck, K. Petritis, K.K. Hixson, W. Qian, M.E. Monroe, and A.M. Mayampurath, et al. 2008.Rapid Sample Processing For LC-MS Based Quantitative Proteomics Using High Intensity Focused Ultrasounds.Journal of Proteome Research 7, no. 9:3860-3867.PNNL-SA-59400.doi:10.1021/pr800161x