April 10, 2003
Journal Article

Purification, crystallization and preliminary X-ray analysis of two Nudix hydrolases from Deinococcus radiodurans

Abstract

Two Nudix hydrolases from Deinococcus radiodurans, DR1025 and DR0079, have been purified and crystallized. Diffraction data have been collected to 1.4 Sand 1.9 Sfor DR1025 and DR0079, respectively. DR1025 belongs to space group P41212/P43212 with cell dimensions a=b=53.22? c=122.57S(Vol. 346,882.9 ?, Vm 2.47?/Dalton, solvent content 50.18%). DR0079 belongs to space group C222 with a=34.25? b=157.36, c=126.41 (Vol. 68,0983.2 ?, Vm 2.21?/Dalton, solvent content 44.27%). The calculated unit cell content suggests that DR1025 is a monomer in the asymmetric unit in agreement with solution results by dynamic light scattering. The space group and cell dimensions of DR0079 indicate two molecules per asymmetric unit, however gel filtration and NMR spectroscopy suggest it is a monomer in solution.

Revised: July 8, 2008 | Published: April 10, 2003

Citation

Holbrook E.L., U. Schulze-Gahmen, G.W. Buchko, S. Ni, M.A. Kennedy, and S.R. Holbrook. 2003. Purification, crystallization and preliminary X-ray analysis of two Nudix hydrolases from Deinococcus radiodurans. Acta Crystallographica. Section D, Biological Crystallography 59, no. Pt.4:737-740. PNNL-SA-37353.