This review will describe developments, primarily from our laboratory, in fundamental and applied aspects of separations, and mass spectrometric instrumentation important for making more effective and quantitative proteomics measurements. The basis for better quantitation and ultra-high sensitivity will be highlighted for high resolution capillary liquid chromatography separations that provide low nL/min flow rates to an electrospray ionization interface. The increased dynamic range of measurements and sub-attomole regime detection limits obtainable open new avenues for proteomics research.
Revised: May 11, 2011 |
Published: December 12, 2006
Citation
Jacobs J.M., and R.D. Smith. 2006.Proteomic Analyses Using High-Efficiency Separations and Accurate Mass Measurements. In Separation Methods in Proteomics, edited by GB Smejkal and A Lazareu. 363-385. Boca Raton, Florida:CRC Taylor & Francis.PNNL-SA-42439.