The coupling of high-performance mass spectrometry instrumentation with highly efficient chromatographic and electrophoretic separations has enabled rapid qualitative and quantitative analysis of thousands of proteins from minute samples of biological materials. Here, we review recent progress in the development and application of mass spectrometry-based techniques for the qualitative and quantitative analysis of global proteome samples. Techniques such as multidimensional peptide and protein separations coupled with mass spectrometry, accurate mass measurement of peptides from global proteome digests, and mass spectrometric characterization of intact proteins hold great promise for characterization of highly complex protein mixtures. Advances in chemical tagging and isotope labeling techniques have enabled quantitative analysis of proteomes, and highly specific isolation strategies have been developed and aimed at selected analysis of post-translationally modified proteins.
Revised: June 29, 2011 |
Published: June 1, 2003
Citation
Ferguson P.L., and R.D. Smith. 2003.Proteome Analysis by Mass Spectrometry. In Annual Review in Biophysics and Biomolecular Structure, edited by Robert M. Stroud. 399-424. Palo Alto, California:Annual Reviews.PNNL-SA-37868.