The objectives of this chapter are to (a) briefly introduce general strategies for culture-independent analysis of community nucleic acids, (b) elucidate some of the challenges, limitations and pitfalls of culture-independent techniques and data interpretation, (c) introduce some possible technological solutions for overcoming these pitfalls, and (d) discuss the potential implications of technology developments for a more comprehensive understanding of biogeochemical processes in (in situ and ex situ) subsurface materials.
Revised: December 16, 2010 |
Published: January 17, 2001
Chandler D.P., and F.J. Brockman. 2001.Nucleic acid analysis of subsurface microbial communities: pitfalls, possibilities, and biogeochemical implications. In Subsurface Microbiology and Biogeochemistry, edited by JK Fredrickson and M Fletcher. 281-313. New York, New York:Wiley and Sons.PNNL-SA-38021.