In this manuscript, we report on electrochemical biosensors based on various nanoparticles (NPs) as labels for sensitive detection of protein biomarkers. We used silica nanoparticle as a carrier to loading a large amount of electroactive species such as poly(guanine) for sensitive immunoassay of tumor necrosis factor-alpha (TNF-a). We took the advantages of the unique hollow structure and reconstruction properties of apoferritin to prepare Cd3(PO4)2 nanoparticles as labels for sensitive assay of TNF-a. A novel immunochromatographic/electro-chemical biosensor based on quantum dots as labels has also been developed for rapid and sensitive detection of prostate-specific antigen (PSA) in human serum. These biosensors are quite sensitive with the detection limit at pM level and these approaches based on nanoparticle labels offer a new avenue for sensitive detection of protein biomarkers.
Revised: November 5, 2008 |
Published: October 12, 2008
Citation
Lin Y., J. Wang, H. Wang, H. Wu, and Z. Tang. 2008.Nanoparticles for Enhanced Sensitivity in Electrochemical Immunoassays. In Chemical Sensors 8: Chemical Sensors and Analytical Systems. ECS Transactions., edited by R. Mukundan, et al, 16, 477-482. Pennington, New Jersey:The Electrochemical Society.PNNL-SA-61565.