Glycan moiety of glycoproteins plays an essential role in its biological activity in vivo, and the analysis of glycosylation is of great importance in the development of protein therapeutics. In this study, we report a rapid and simple detection of protein glycosylation based on the fluorescence resonance energy transfer (FRET) between concanavalin A-conjugated gold nanoparticles (ConA-AuNPs) and dextran-conjugated quantum dots (Dex-QDs). The increased photoluminescence (PL) signals of Dex-QDs due to the competitive inhibition of glycoproteins were well correlated with the glycosylation chain length of glucose oxidases as well as the mannosylation degree of bovine serum albumin (BSA). The parallel analysis of the diversely mannosylated BSAs using an image analyzer further demonstrated the potential of this new technique in high-throughput screening of glycoprotein and carbohydrate therapeutics.
Revised: January 30, 2007 |
Published: December 4, 2006
Citation
Oh E., D. Lee, Y. Kim, S.Y. Cha, D.B. Oh, J. Kim, and H.A. Kang, et al. 2006.Nanoparticle-based energy transfer for rapid and simple detection of protein glycosylation.Angewandte Chemie International Edition 45, no. 47:7959-7963.PNNL-SA-49712.doi:10.1002/anie.200601948