July 22, 2020
Journal Article

Multi-platform 'Omics Analysis of Human Ebola Virus Disease Pathogenesis

Pete Halfmann
Jason Wendler
Jennifer Kyle
Amie Eisfeld
Zuleyma Peralta
Tadashi Maemura
Cameron Casey
Kevin Walters
Gabriele Neumann
Jennifer Reed
Alhaji N'jai
Thomas Metz
Katrina Waters
Foday Sahr
Yoshihiro Kawaoka
Tokiko Watanabe
Jon Jacobs
Young-Mo Kim
Kelly Stratton
Satoshi Fukuyama
Makoto Yamashita
Marina Gritsenko
Karl Weitz
Anil Shukla
Mingyuan Tian
Harm van Bakel

Abstract

The pathogenesis of human Ebola virus disease (EVD) is complex. EVD is characterized by high levels of virus replication and dissemination, dysregulated immune responses, extensive virus- and host-mediated tissue damage, and disordered coagulation. To clarify how host responses contribute to EVD pathophysiology, we performed multi-platform ’omics analysis of peripheral blood mononuclear cells and plasma from EVD patients. Our results indicate that EVD molecular signatures overlap with those of sepsis, imply that pancreatic enzymes contribute to tissue damage in fatal EVD, and suggest that Ebola virus infection may induce aberrant neutrophils whose activity could explain hallmarks of fatal EVD. Moreover, integrated biomarker prediction identified putative biomarkers from different data platforms that differentiated survivors and fatalities early after infection. This work reveals insight into EVD pathogenesis, suggests an effective approach for biomarker identification, and provides an important community resource for further analysis of human EVD severity.

Revised: July 22, 2020 | Published: December 13, 2017

Citation

Eisfeld A.J., P. Halfmann, J.P. Wendler, J.E. Kyle, K.E. Burnum-Johnson, Z. Peralta, and T. Maemura, et al. 2017. "Multi-platform 'Omics Analysis of Human Ebola Virus Disease Pathogenesis." Cell Host & Microbe 22, no. 6:817-829. PNNL-SA-120881. doi:10.1016/j.chom.2017.10.011