October 8, 2002
Conference Paper

Microsphere Capture and Perfusion in Microchannels Using Flexural Plate Wave Structures

Abstract

A standing acoustic field excited by an ultrasonic flexural plate wave (FPW) device is shown to trap microspheres and cells suspended in a pressure-driven flowing liquid. Capture is achieved by counteracting the viscous drag forces on a particle with acoustic radiation pressure. The suitability of this technique for biochemical analysis is demonstrated with tow experiments: (1) acoustically trapped streptavidin-coated 1 _m microspheres conjugated to fluorescent 200 nm biotinylated microspheres; and (2) perfusion of the membrane permeant fluorescein diacetate across acoustically trapped cells. Biochemical interaction was monitored with a fluorescence microscope. Efforts to integrate acoustic traps with on-chip FPW microfluidic pumps are also described.

Revised: June 21, 2006 | Published: October 8, 2002

Citation

Black J., R.M. White, and J.W. Grate. 2002. Microsphere Capture and Perfusion in Microchannels Using Flexural Plate Wave Structures. In Proceedings of the IEEE Ultrasonics Symposium, 1, 475-479. Piscataway, New Jersey:Institute of Electrical and Electronic Engineers. PNNL-SA-43435.