Direct liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to examine the proteins extracted from Desulfovibrio vulgaris cells. While our previous study provided a proteomic overview of the cellular metabolism based on proteins with known functions (Zhang et al., 2006a, Proteomics, 6: 4286-4299), this study describes the global detection and functional inference for hypothetical D. vulgaris proteins. Across six growth conditions, 15,841 tryptic peptides were identified with high confidence. Using a criterion of peptide identification from at least two out of three independent LC-MS/MS analyses per protein, 176 open reading frames (ORFs) originally annotated as hypothetical proteins were found to encode expressed proteins. These proteins ranged from 6.0 to 153 kDa, and had calculated pI values ranging from 3.7 to 11.5. Based on homology search results (with E value
Revised: October 1, 2015 |
Published: November 3, 2006
Citation
Zhang W., D.E. Culley, M.A. Gritsenko, R.J. Moore, L. Nie, J.C. Scholten, and K. Petritis, et al. 2006.LC-MS/MS Based Proteomic Analysis and Functional Inference of Hypothetical Proteins in Desulfovibrio Vulgaris.Biochemical and Biophysical Research Communications 349, no. 4:1412-1419.PNNL-SA-51397.doi:10.1016/j.bbrc.2006.09.019