The use of Layer-by-layer techniques for immobilizing several types of enzymes, e.g. glucose oxidase (GOx), horse radish oxidases(HRP), and choline oxidase(CHO) on carbon nanotubes and their applications for biosenseing are presented. The enzyme is immobilized on the negatively charged CNT surface by alternatively assembling a cationic polydiallyldimethyl-ammonium chloride (PDDA) layer and a enzyme layer. The sandwich-like layer structure (PDDA/enzyme/PDDA/CNT) formed by electrostatic assembling provides a favorable microenvironment to keep the bioactivity of enzyme and to prevent enzyme molecule leakage. The morphologies and electrocatalytic acitivity of the resulted enzyme film were characterized using TEM and electrochemical techniques, respectively. It was found that these enzyme-based biosensors are very sensitive, selective for detection of biomolecules, e.g. glucose, choline.
Revised: October 20, 2009 |
Published: June 1, 2008
Citation
Wang J., G. Liu, and Y. Lin. 2008.Layer-by-Layer Assembly of Enzymes on Carbon Nanotubes. In Biomolecular Catalysis: Nanoscale Science and Technology, edited by J Kim, SH Kim, P Wang. 117-128. Washington Dc:American Chemical Society.PNNL-SA-53316.