February 16, 2024
Book Chapter

Ion Mobility Spectrometry-Mass Spectrometry for High-Throughput Analysis


Ion mobility spectrometry is a widely used analytical technique providing gas phase separation of molecules. It has received increasing attention in the recent years with the advancement in technology development and the availability of commercial instruments. In this chapter, we introduced the ion mobility fundamental theory and provided examples of IMS applications, especially for isomer separation, collision cross section database generation, high throughput analysis workflows, software tools for IMS data analysis, and ongoing high resolution SLIM IMS development. While IMS is not yet routinely utilized in drug discovery and pharmaceutical industry, there has been increased interest in high throughput library screening and antibody characterization. With all the ongoing development in IMS technology and informatics, we foresee more and more exciting applications of high throughput IMS analysis in different fields including omics studies, drug discovery and clinical applications in the near future.

Published: February 16, 2024


Ross D.H., A. Bilbao, R.D. Smith, and X. Zheng. 2023. Ion Mobility Spectrometry-Mass Spectrometry for High-Throughput Analysis. In High-Throughput Mass Spectrometry in Drug Discovery, edited by C. Liu and H. Zhang. 183-213. Hoboken, New Jersey:John Wiley & Sons, Inc. PNNL-SA-177811. doi:10.1002/9781119678496.ch6

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