The goal of metabolomics experiments is the detection and quantitation of as many sample components as reasonably possible in order to identify “features” that can be used to characterize the samples under study. When utilizing electrospray ionization to produce ions for analysis by mass spectrometry (MS), it is imperative that metabolome sample constituents be efficiently separated prior to ion production, in order to minimize the phenomenon of ionization suppression. Similarly, optimization of the MS inlet can lead to increased measurement sensitivity. This review will focus on the role of high resolution liquid chromatography (LC) separations in conjunction with improved ion production and transmission for LC-MS-based metabolomics.
Revised: March 5, 2009 |
Published: March 31, 2008
Citation
Metz T.O., J.S. Page, E.S. Baker, K. Tang, J. Ding, Y. Shen, and R.D. Smith. 2008.High Resolution Separations and Improved Ion Production and Transmission in Metabolomics.Trends in Analytical Chemistry. TrAC 27, no. 3:205-214.PNNL-SA-56841.doi:10.1016/j.trac.2007.11.003