It is demonstrated that the resolution of the 1H NMR metabolite spectrum in a live mouse can be significantly enhanced by an ultra-slow magic angle spinning of the animal combined with a modified phase-corrected magic angle turning (PHORMAT) pulse sequence. Proton NMR spectra were measured of the torso and the top part of the belly of a female BALBc mouse in about one hour in a 2T field, while spinning the animal at a speed of 1.5 Hz. It was found that even in this relatively low field with PHORMAT an isotropic spectrum is obtained with line widths that are a factor 4.6 smaller than those obtained in a stationary mouse. It is concluded that in vivo PHORMAT has the potential to significantly increase the utility of 1H NMR spectroscopy for biochemical and biomedical animal research.
Revised: June 29, 2011 |
Published: December 3, 2003
Citation
Wind R.A., J. Hu, and D.N. Rommereim. 2003.High Resolution H-1 NMR Spectroscopy in a Live Mouse subjected to 1.5 Hz Magic Angle Spinning.Magnetic Resonance in Medicine 50, no. 6:p. 1113-1119.PNNL-SA-38565.